Click [here](https://img1.wsimg.com/blobby/go/c616bfea-edff-477a-b8fb-dc0f66310f31/PRNANO%20issue%203(4)%20Cover%20new.png-5f833f5.pdf) to download a hi-res image of the cover page. ##FEATURE ARTICLE ElSayed MEH, Prud’homme RK, et al., Co-encapsulation by Flash NanoPrecipitation of Insulin, Trypsin Inhibitor and Caprate Permeabilization Enhancer for Oral Administration, [Precis. Nanomed. 2020;3(4):710-723](https://doi.org/10.33218/001c.18519) POTENTIAL CLINICAL SIGNIFICANCE _From the Clinical Editor:_ The delivery of small molecule drugs like peptides and proteins via the oral route is often limited by drug absorption across the intestinal mucosa and enzymes’ digestion. A common strategy to tackle the problems is by drug encapsulation. In this study, the authors presented a new two-step encapsulation technique - “inverse-Flash NanoPrecipitation (iFNP).” They further demonstrated its application using insulin. It is hoped that this customizable technique would be easily adopted in the delivery of other drugs. [ALTMETRIC and CITATIONS](https://precisionnanomedicine.org/prnano-572020) **Opinion** Erden YJ, and Lévy R, The long life of unicorns, [Precis. Nanomed. 2020;3(4):677-684](https://doi.org/10.33218/001c.17635) BASIC SCIENCE _From the Clinical Editor:_ This article is not the usual kind seen in a scientific journal – it’s been written from a philosophic angle. Here, the unicorn is used as an analogy to the sustenance of scientific beliefs and misconceptions, especially in the world of nanoparticles. In this respect, we are reminded by the authors of the importance of scientific rigor. [ALTMETRIC and CITATIONS](https://precisionnanomedicine.org/prnano-542020) **Letter to the Editor** Fadeel B, Comment on “The long life of unicorns,” [Precis. Nanomed. 2020;3(4):709](https://doi.org/10.33218/001c.18251) BASIC SCIENCE Summary: Misconceptions may persist in the scientific literature, and we should not mistake currently prevailing scientific paradigms for the “truth.” Instead, our experimental observations should guide us. [ALTMETRIC and CITATIONS](https://precisionnanomedicine.org/prnano-602020) **Research Articles** Silverman L, and Barenholz Y, Effect of major tumor metabolites on the release of doxorubicin from Doxil – implications for precision nanomedicine, [Precis. Nanomed. 2020;3(4):699-708](https://doi.org/10.33218/001c.17956) CLINICAL SIGNIFICANCE _From the Clinical Editor:_ Advances in nanomedicine have provided drugs for use in the clinical setting, with one of the most well-known being Doxil. Despite this, many underlying mechanisms which lead to the release of active drug in the target location are still not elucidated. The authors here investigated the role of glutaminolysis in the tumor environment in doxorubicin release from Doxil. The data provided here could provide new insights into future drug design into more personalized medicine. [ALTMETRIC and CITATIONS](https://precisionnanomedicine.org/prnano-562020) d’Amora M, Raffa V., et al., Heat-sensitive poly-acrylamide nanoparticle for cancer treatment, [Precis. Nanomed. 2020;3(4):666-676](https://doi.org/10.33218/001c.17629) POTENTIAL CLINICAL SIGNIFICANCE _From the Clinical Editor:_ In the present paper, the authors describe the development of new thermosensitive nanoparticles encapsulating doxorubicin and functionalized with folic acid to improve selective targeting. Applying local hyperthermia (at 41°C) results in a quick release of the drug in vitro and in vivo and offers a promising platform for the treatment of solid tumors. [ALTMETRIC and CITATIONS](https://precisionnanomedicine.org/prnano-552020) Souto EB, Severino P, et. al., Perillyl Alcohol in Solid Lipid Nanoparticles: Cytotoxicity and Antitumor Potential in Sarcoma 180 Mice Model [Precis. Nanomed. 2020;3(4):685-698](https://doi.org/10.33218/001c.17880) POTENTIAL CLINICAL SIGNIFICANCE _From the Clinical Editor:_ In this work, the development of solid lipid nanoparticles encapsulating perillyl alcohol as promising nanocarriers for cancer treatment are described. These particles did not show in vitro any cytotoxicity on non-tumoral cells and demonstrated a significant tumor inhibition in sarcoma transplanted mice without adverse side effects in respective organs, [ALTMETRIC and CITATIONS](https://precisionnanomedicine.org/prnano-582020)

Effect of major tumor metabolites on release of doxorubicin from Doxil – implications for precision nano-medicine.